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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

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HMGB1 is a ubiquitously expressed nuclear protein engaged in the progression of nucleosome stabilization and DNA binding

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