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Products were quantified using Quant-it PicoGreen dsDNA Assay Kit (ThermoScientific) in a Light Cycler 480 II real-time PCR machine (Roche)

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Ramirez MD is a consultant for Mirum, a consultant for Soleno Theraputics, and is on the board of directors for Global Foundation of Peroxisomal Disorders

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