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In endothelial cells, the ERK1/2 pathway is activated in a Ca 2+ -dependent manner 48,49 and ERK1/2 activation exacerbates renal fibrosis 50

Il1b is a well-established negative regulator of insulin signaling, primarily exerting its effects by downregulating the IRS1/PI3K/Akt axis

To construct the shRNA knockdown vector pLKO.1-GST DAF , we inserted the GST-EGFP-GPI DAF coding region into the pLKO.1 puro plasmid in place of the puromycin resistance gene