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Dual-luciferase assay HEK293T cells were transfected with 10 ng each of psiCHECK-2 construct along with 15 pmol of the miR-483-3p mimic or an identical amount of the control mimic with Lipofectamine 2000 (Invitrogen)

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Regulatory limits on BPA exposure, and practical considerations for reducing exposure [1:12:45]