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The quantification of GLP-1 levels in tissue extracts was normalized to the protein levels of tissue lysates, which were determined using a BCA Protein Assay Kit (Thermo Fisher Scientific, #A55860)

Additionally, neither male (Supplemental Figure 1, KP) nor female (Supplemental Figure 1, QV) heterozygous Bbs5 +/ mice displayed any discernable phenotypic changes compared with WT controls

demonstrated Thymosin Beta-4's ability to promote cardiac cell migration, survival, and repair

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