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Once the samples reached confluency the cells were subcultured and all tendon fibroblasts between passages 2 and 4 that had a proper growth rate and normal fibroblast shape were used in the experimentation process

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When breaks are mandatory Must stop if : Severe side effects develop Health markers worsen Doctor recommends discontinuation Pregnancy or trying to conceive Should take break if : Results have plateaued despite dose increases Using very long continuous protocols (9+ months) Need financial break Experiencing injection fatigue Break duration : 4-8 weeks typically sufficient What happens during break : Benefits gradually decline Body returns closer to baseline Can restart with renewed response Strategic breaks vs forced breaks Strategic breaks (planned): Schedule every 12-16 weeks Part of overall protocol Budgeted into timeline Use to reassess goals Forced breaks (reactive): Side effects or issues Financial constraints Life circumstances Less ideal but necessary Better approach : Plan breaks proactively Transitioning between peptides Smooth transitions optimize results