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i , Immunoblot analysis of SYVN1 from SLC33A1 -knockout HEK293T cells complemented with a vector control or SLC33A1 cDNA expressing sgRNA targeting intergenic region or two different sgRNAs targeting SYVN1

In brief, total RNA was extracted using TRIzol Reagent (Invitrogen) according to manufacturers protocol

Transcription factors such as NLP7 , PHR1 , and bZIP family members coordinate nutrient assimilation and transport, while specific regulators like IDEF1/IDEF2 and bZIP19/bZIP23 provide targeted responses to iron and zinc deficiencies, highlighting opportunities for biofortification and improved nutritional quality

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