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In various cell systems, such signal activation downstream of ECM cues has been reported to affect the localization and activation levels of various transcription factors and coregulatory factors, such as Yes-associated protein (YAP), leading to gene expression changes that impact processes like proliferation, survival, or differentiation (Ishihara and Haga, 2022)
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For STED microscopy, Goat-Anti-Rabbit-IgG-Atto 647 N (Sigma-Aldrich) and Goat anti-Mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 594 (Thermo Fisher Scientific) were used at the 1:100 dilution
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