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The mixture was incubated at 50C for 5 min

Thus, the right-hand side of the upper equation can be calculated

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Recombinant antigens are used to capture circulating anti-HCV antibodies onto the wells of microtiter plates, microbeads, or specific holders adapted to closed automated devices

When designing a KLOW 80mg protocol, researchers calculate the working concentration from their chosen reconstitution volume and then determine per-use volumes from there