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Although comprehensive exercise showed the largest effect size, high heterogeneity and few studies limit its reliability

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Methods Systems construction for MD-simulation The open form of NADH- and GTP bound GDH system (PDB id: 3jd3) cryo-EM structure was chosen and ligands, water molecules, other solvent and unwanted heterogens were solved and removed from structure by PDBFixer 30 at pH 6.8