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Figure 4 Figure 5 ENDS exposure induces lipid dysregulation and ferroptosis in mice We noticed that the level of cell death detected by TUNEL staining (either apoptosis or necrosis) does not fully match with lung tissue destruction by ENDS as shown in histological staining

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Evidence indicates that dual users tend to maintain consumption of both products over time 74

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Discussion Given the critical role of mitochondrial function in the pathophysiology of COPD and its reported association with nDNA methylation, we set out to explore the effect of cigarette smoke exposure on DNA methylation of the mitochondrial genome

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