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In vitro harvest for scRNA-seq (WaferGen) For each timepoint, medium was removed from endpoint ALI cultures, and the apical chamber was washed with warm PBS/DTT (10 mM) for 5 min at 37 C, followed by a warm PBS wash of both chambers
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Doctors have said the illnesses resemble an inhalation injury, with the lungs apparently reacting to a caustic substance
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