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The recommended number of cartridges is 616, with each cartridge used in three smoking periods

Using these datasets, an overlap was found in gene expression related to oxidative stress, xenobiotic metabolism, AhR and Nrf2 pathways, as shown by a positive association between the in-vitro study and the former two in-vivo smoker studies, and a negative association with the in-vivo smoking cessation study (Additional file 2: Table S3)

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Massive PINK1 accumulation on OMM leads to hyperactivation of mitophagy, resulting in programmed cell death