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In order to obtain the generation of full-length viral cDNA sequence, TMV-RNA was reverse transcribed using primer cDNA (Table 1) in 50 mmol/L Tris at pH 8.0, 8.0 mmol/L magnesium chloride, 75 mmol/L potassium chloride, 10 mmol/L DL-Dithiothreitol, 1.0 mmol/L dNTPs, 0.5 unit/L AMV reverse transcriptase (TaKaRa), and 1.0 unit/L RNase inhibitor (TaKaRa) for 1.5 hr at 315 K