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From writers and philosophers to politicians and entertainers, these individuals have left a lasting legacy, in part due to their association with the iconic image of the pipe smoker

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Membranes were homogenized in a solution of 50 mM HEPES pH 7.3, 300 mM NaCl, 20 mM Imidazole, 20 mM DDM and 0.5 mM Tris-(2-Carboxyethyl)phosphine (TCEP), then allowed to solubilise for 4 h at 4 C