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Western blot analysis showed that, compared with the CSE treatment group, the expressions of Sp1 and SIRT1 in the cells transfected with the miR-125a-5p inhibitor increased, the level of HIF-1 acetylation decreased, and the cell senescence markers p21 and p27 were down-regulated

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Since the expression of miR-320d was not different between males and females, the gender imbalance between the cohorts not likely contributes to the observed discrepancies

The whole trachea tissue samples were lysed in 250 l of Lysis Buffer with Triton X-100 by sonication

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