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Different from exosomes, the ectosomes, including microvesicles and microparticles, are ubiquitous vesicles directly released from the plasma membrane through outward budding with a size range of 50 nm1 m in diameter [31, 32] (Fig

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Then, 10 mL of deionized water was added to the tube, and the mixture was extracted in a 60 C water bath for 30 min

The calculation steps were as follows

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