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Longitudinal studies integrating metagenomic, transcriptomic, metabolomic, methods with clinical results may help us ascertain the relationships between smoking, microbiome, and pathological mechanism in diseases

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Cigarette smoke is a well-established risk factor in the development of chronic obstructive pulmonary disease (COPD), which is characterized by chronic airflow limitation associated with various extra-pulmonary manifestations, such as cardiovascular, metabolic, diabetic, muscular and bone disorders 4