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Cell pellets resuspended in BEGM + 0.5 PSA were filtered using a 70 m cell strainer, collected by centrifugation (5 min, 225 g , 4 C) and cryopreserved in freeze media (F-media, 30% FBS, 10% DMSO)

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by impaction or diffusion) 24 depends on the aerosol particle characteristics such as aerodynamic and mobility diameters, density, and volatility 25,26,27,28

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Subsequently, a laboratory study confirmed that elevating power levels leads to increased aerosol yield and nicotine delivery from the liquid to the aerosol 13