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Immunofluorescence staining and imaging Cells in AX12 were fixed using 4% paraformaldehyde in distilled PBS- (PBS without calcium and magnesium)

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After the saliva is swallowed, it neutralizes acid in the esophagus

Some promising approaches that are being evaluated include: Sequential behavioral treatment (cessation, then diet and activity change) more effective than simultaneous treatment because it is less overwhelming to the patient