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Interestingly, the alkaloid content was minimally impacted in the CLCNt2 -RNAi lines (Figure 4A)

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We have shown previously that a dramatic reduction in the nitrate content in BU leaves can be realized through constitutive expression of a nitrate reductase enzyme that can no longer be downregulated via reversible phosphorylation, this constitutive expression is achieved through substitution of an Asp residue for Ser523 (Lu et al., 2016, 2021)