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solanacearum 11 was grown in Nutrient broth (NB, beef extract 3.0 g/L, peptone 10 g/L, NaCl 5 g/L pH 7.47.6) overnight and the cells (OD 600 = 1.0) were centrifuged, washed twice with phosphate buffer (100 mM, pH 7.0), and then resuspended in NB (OD 600 = 1.0)