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Dannielle Green, a marine ecologist at Anglia Ruskin University in the U.K., reviewed the evidence of cigarette butt pollution in terrestrial and aquatic environments, and she underlines the potentially lethal and sublethal impacts these can have on species: Theres evidence for reproductive rate effects, changes in growth, malformations and developmental impacts. Research has shown nicotine and other chemicals in cigarette butts are toxic to microbes, plants, benthic organisms, bivalves, zooplankton, fish, and mammals, but warn that critical knowledge gaps remain

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The first group was treated with 1 M daunorubicin, a fluorescent chemotherapeutic substrate for P-gp and MRP1