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Samples were stored at 80C until used for immunoblotting

Although consumption of filtered and non-filtered cigarettes decreased from 465 million packs in 2016 to 216 million packs by 2019, cigarette consumption rose once again during the pandemic and reached 291 million packs in 2021

The expansion was largely driven by the Asia-Pacific region, particularly China and Hong Kong

This supports the theory that bupropion does not modify neurotransmitter activity and has no longer-term effects beyond the treatment period. 4 Bupropion is sometimes prescribed in combination with other non-nicotine therapies, like varenicline (Chantix)