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Analysis of protein expression by flow cytometry Cells were fixed in 4% paraformaldehyde in PBS (pH 7.4) for 20 min at room temperature and permeabilized in 0.1% Triton X-100 in PBS with 0.1% FBS for 5 min

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Chemotherapy is not given every day but instead is given in cycles

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A: More than 2000COTS cells are available

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