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Mitochondrial content was assessed by using flow cytometry for MitoTracker Green FM that fluoresces independently of mitochondrial [Ca 2+ ] and membrane potential, real-time PCR to quantify mtDNA content, and immunoblotting for GRP75 (mitochondrial chaperone, matrix protein) and NDUFA9 (complex I subunit, inner membrane protein)
For more on urban workers, see: Ira Berlin, Many Thousands Gone: The First Two Centuries of Slavery in North America (1998)
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