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A rep for JSX, the public charter operator for the plane, previously told Page Six, We have no comment regarding the alleged events described by TMZ. Spears reps could not be reached for comment.

In vitro harvest for scRNA-seq (WaferGen) For each timepoint, medium was removed from endpoint ALI cultures, and the apical chamber was washed with warm PBS/DTT (10 mM) for 5 min at 37 C, followed by a warm PBS wash of both chambers