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Western Blot Analyses The liver tissue was homogenized using T-PER Tissue Protein buffer (Thermo Fisher, 78510), supplemented with protease inhibitors (Thermo Fisher, #87785) and proteins were separated by polyacrylamide electrophoresis and transferred onto nitrocellulose membrane as described ( NAD+/NADH Ratio Determination in Hepatocytes Assessment of NAD+ and NADH and their ratio was performed using a quantitative colorimetric assay (Bio Assay systems, #E2ND-100), according to manufacturer's instructions

Excess risk of heart disease is reduced by half after one years abstinence

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