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The alkylation reaction was quenched by adding 500 mM DTT solution to a final concentration of 10 mM

Heres how smoking affects female fertility: DNA Damage: Chemicals in cigarettes can cause genetic abnormalities in eggs

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PCR of genomic DNA was conducted using a 2-ng l 1 template, 0.25 M of each primer and GoTaq Hot Start polymerase (Promega AG, Switzerland)

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