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Also, few women use chewing tobacco, compared to men: 18% of men used it in the past 30 days compared to 1% of women
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Next, specimens were dehydrated through an ascending ethanol series, starting with 50% ethanol, followed by incubation in 70% ethanol, 1% uranyl acetate (#21447, Polyscience Europe, Heidelberg, Germany), and 1% phosphotungstic acid (#455970, Merck, Darmstadt, Germany) solution overnight at 4 C
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