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The sample is subjected to reversed phase liquid chromatography and quantitated via fluorescence detection

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People with Li-Fraumeni syndrome that inherit one mutant p53 allele and one WT p53 allele have an ~50% likelihood of developing cancer by the age of 35 and a ~90% risk of developing cancer throughout their lifetime [83]

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To open it, you tear off the top part of the wrapping along the perforated line, lift off the blue, plastic thingy, remove the silver foil seal thats covering the charcoal inside, then place the blue, plastic thingy back