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Table 4 shows a negative but nonsignificant total indirect effect (-0.007), alongside negative (and nonsignificant) single-mediator indirect effects through M1 (-0.005) and M2 (-0.003), yet a positive and significant serial component (0.001)

This result can be partially explained by the fact that the half-life of cotinine is ~17 hours in human and ~1 hour in mice 7,22

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