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For the microbiome analysis, deoxyribonucleic acid (DNA) analysis was conducted at the end of the study on a single fecal pellet from each mouse, and the 16s ribosomal ribonucleic acid (rRNA) gene was amplified from the extracted DNA

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Over the past five decades, there has been a remarkable reduction in smoking in Canada: approximately half of Canadians smoked in 1965, compared to just one in ten in 2020 (Figure 1.1)

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[69] It is ideal to balance known hormonal issues as much as possible before conception, and monitor them closely during pregnancy and after birth

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