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The precipitated pellet was then mixed with sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) sample loading buffer and analyzed by immunoblotting

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As an example, an individual who smoked 20 cigarettes a day for 15 years but then did not smoke for 17 years had more than twice the odds of developing schizophrenia and almost twice the odds of developing depression than someone who had never smoked