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Previous ICSS studies have observed that chronic exposure to drugs sensitizes the brain reward system, and in doing so lowers the stimulation threshold (expressed as a current intensity or stimulation frequency) 46 required for ICSS 47

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However, a number of pathological and physiological factors, such as taste and smell stimulation, genetics, hormonal status, and dental hygiene, can affect the amount and quality of saliva produced in human mouth (Roberts and Johnson 2012)