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Lohan was reportedly hesitant to film the scenes because of Sheen's party boy rep and was apparently afraid she might catch something from him if they locked lips

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Ouchterlony O (1958) Diffusion-in-gel methods for immunological analysis

Then, the immunoprecipitated materials were separated by SDS-PAGE and subjected to western blot analysis with anti-SP-A antibody as described above

Sci Rep 12 , 20336 (2022)