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Mitochondrial oxidation of fatty acids, glucose, and glutamine were determined by the Seahorse Analyzer following the instruction of the Seahorse XF Mito Fuel Flex Test kit ( Mass Spectrometry Analysis To directly measure fatty acid oxidation (FAO), cells were incubated with medium supplemented with 100 M [U- 13 C] palmitate or palmitate conjugated to BSA for 6 h (Yao et al., 2016)