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Bacteria were grown in TB broth at 37 C to an OD 600 of 1.2, and protein expression was induced with the addition of 0.2 mM of isopropyl -d-1-thiogalactopyranoside (IPTG)
2 mg/mL concentration A mid-range concentration that balances accuracy with convenience
*Trebaticka, J., et al., Treatment of ADHD with French maritime pine bark extract, Pycnogenol
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