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qPCR analysis confirmed that Rf regulates critical genes, including ANXA2, BAZ1A, DNMT3L, and MMP9, thereby clarifying its underlying mechanism of action

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The comparatively higher sample size addresses biological variability, possible attrition and death rates (e.g., Doxorubicin toxicity), and type I error inflation in pairwise comparisons (Tukeys post-hoc test)

NAC may be a better choice in these cases, as it contains less sulfur overall

5 Adverse effects Progoitrin and indole glucosinolates in Brassica vegetables can degrade to goitrin in the presence of myrosinase, potentially decreasing thyroid hormone production (144146)