Vitamin B12 is a water-soluble vitamin primarily found in animal-based foods such as red meat, fish, poultry, eggs, and dairy products
After fatty acid treatment, the organoids were incubated with 3.8 M BODIPY 493/503 (Life Technologies) for 30 min in culture medium at 37 C and then washed two times with PBS before being replaced with fresh culture medium containing DRAQ5 (Thermo Fisher) at 1:1500
But if the typical vitamin B12 sources are not part of your regular diet or if your body has difficulty absorbing enough B12, there are other options
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Johnson, J
A 12-week randomized controlled trial in vegans and vegetarians with marginal deficiency (Effect of two different sublingual dosages of vitamin B12 on cobalamin nutritional status, Del Bo and colleagues, Clinical Nutrition , 2018, 40 subjects) found that even a modest sublingual dose of about 50 mcg per day restored serum B12 and improved holotranscobalamin, methylmalonic acid, and homocysteine, which confirms that sublingual liquid B12 can correct a marginal deficiency in plant-based eaters