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It's the first checkpoint for data reliability

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They also represented a departure from FDA staff recommendations against inclusion, creating one of the most closely watched peptide developments in years

Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h

Shaking instead of swirling Vigorous shaking creates foam and mechanical stress that damages the peptide

Which means the antioxidant capacity is compromised